Name:  Eman Samir Ramis               

Date of birth:   11-11-1973.

 Nationality: Egyptian

Previous Degree:  B.Sc.

Registration Date: April 2003

Awarding Date:  December 2007

Specialization:  cytology, histology and Genetics.

Supervisors: - Prof. Dr. Mahmoud Ahmad Amer

                    - Dr. Tarek Darder Hussein

                    - Prof. Dr. Akila Saleh Hamza

Examiners:- Prof. Dr. Günter Schmahl

                    - Prof. Dr. Lucia Migliore 

 -                    Prof. Dr. Mahmoud Ahmad Amer

Title of Thesis:

        Histopathological, histochemical and ultrastructural effects of coloring agents as food additives on some mammalian organs.

Key Words: -

Mammals, mice, liver, kidney, histology, histochemistry, ultrastructure, polysaccharides, proteins and nucleic acids.

ABSTRACT:

The effect of erythrosine (a food coloring agent) on the liver and kidney of mice was studied through treating 7 groups of animals with 680 mg/kg b. wt. and examining them at different intervals (o, 1, 2, 4, 6, and 8 weeks) and testing the recovery after one month of the last dose. Erythrosine induced marked histological, histochemical and ultrastructural changes in the liver and kidney tissues, these changes were time dependent. Erythrosine caused cytoplasmic vacuolation, nuclear pyknosis, tissue necrosis, fibrosis and infiltration of inflammatory cells. It also caused disruption of plasma membranes, swelling of mitochondria and fragmentation of endoplasmic reticulum. This was associated with increased number of lysosomes. These changes were reflected on some cytoplasmic inclusions such as polysaccharides, which showed a gradual decrease in both liver and kidney cells. Also there was a noticeable reduction followed by an increase in the protein content of hepatocytes and general reduction of the protein content in the treated kidney. The nucleic acids showed a general reduction in the liver and increase in the kidney after 8 weeks of treatment. Signs of recovery in the liver and kidney tissues after one month of the last dose were seen in the histological and histochemical features. This study indicates that erythrosine is dangerous at continual high doses and it must be avoided especially in case of patients with hepatic and renal disorders.

 

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